MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT P2Y11 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human P2RY11 cDNA (GenBank Accession Number NM_002566) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: P2Y purinoceptor 11 encodes P2Y11 (P2Y11) which is a receptor for ATP and ADP coupled to G-proteins that activate both phosphatidylinositol-calcium and adenylyl cyclase second messenger systems. P2Y11 receptor is unique amongst P2Y receptors with its coupling to the adenylyl cyclase pathway. Through this mechanism, released nucleotides can increase in P2Y11-containing cells. P2Y11 receptor has been reported to be involved in the granulocytic differentiation of promyelocytes and in the maturation of monocyte-derived dendritic cells.
Application: Functional assays
Figure 1. Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Torres et al. (2002) P2Y11 receptors activate adenylyl cyclase and contribute to nucleotide-promoted cAMP formation in MDCK-D(1) cells. A mechanism for nucleotide-mediated autocrine-paracrine regulation. J Biol Chem 277:7761-7765.
Schnurr et al. (2003) ATP gradients inhibit the migratory capacity of specific human dendritic cell types: implications for P2Y11 receptor signaling. Blood 102:613-620.