MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT PK2 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C- 6164)
Expression vector containing full-length human PK2 cDNA (GenBank Accession Number NM_144773.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM-F12, 10% FBS, 10 μg/ml puromycin
Stable in culture for minimum of two months
Background: Prokineticin receptor 2 (PK2 or GPR73L1) is a receptor for the cysteine-rich secreted peptides prokineticin 1 and 2. RT-PCR detected PK2 expression in the brain, testis, small intestine, ovary, thyroid, pituitary and salivary gland.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Lin et al. (2002) Identification and molecular characterization of two closely related G protein-coupled receptors activated by prokineticins/endocrine gland vascular endothelial growth factor. J Biol Chem 277:19276-19280.