MULTISCREEN™ STABLE CELL LINES
HUMAN RECOMBINANT LPA2 (EDG4) RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human EDG4 cDNA (GenBank Accession Number NM_004720.4) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 5 μg/mL puromycin
Stable after minimum of two months continuous growth
Background: The lysophosphatidic acid receptor LPA2 or endothelial differentiation, G-protein coupled receptor 4(EDG-4) is expressed most abundantly in testes and peripheral blood leukocytes. It is reported to be a distinctive functional marker for ovarian carcinoma.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.
Huang et al. (2002) Lysophospholipid mediators of immunity and neoplasia. Biochim Biophys Acta 1582:161-167.
An et al. (1998) Characterization of a novel subtype of human G proteincoupled receptor for lysophosphatidic acid. J Biol Chem 273:7906-7910.