MULTISCREEN™ STABLE CELL LINES

RAT RECOMBINANT UT RECEPTOR

Product Information

Catalog Number:
C1035-1

Lot Number:
C1035-1-073005

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO dhfr-

Transfection:
Full-length rat Uts2r cDNA (GenBank Accession Number U32673)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: Alpha-MEM (Hyclone: SH30265), 10% FBS, 800 μg/mL G418

Stability:
Stable in culture for minimum of two months

Data Sheet

Background: The urotensin 2 receptor (UT or GPR14) is high affinity receptor for urotensin-2 and urotensin-2B. In human, Urotensin II is the most potent vasoconstrictor known, possibly contributing to several human cardiovascular diseases. Both UT receptor and urotensin II are widely expressed in many other tissues, implicating urotensin II in the pathogenesis of a variety of disease processes ranging from hypertension to hepatic cirrhosis and UT antagonists for the possibility of a new range of therapeutic drugs.

Application: Functional assays

Figure Legend. Dose-dependent stimulation of calcium flux upon treatment with ligand, monitored with FlexStation.

References:

Kemp et al. (2005) Urotensin II: a vascular mediator in health and disease. Curr Vasc Pharmacol 3:159-168.

Carotenuto et al. (2004) Urotensin-II receptor peptide agonists. Med Res Rev 24:577-588.

FOR RESEARCH USE ONLY.
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