MULTISCREEN™ MEMBRANE PREPARATION

RECOMBINANT SMOOTHENED RECEPTOR

Product Information

Catalog Number:
MH1442

Lot Number:
MH1442-021317

Quantity:
1 Vial (8.3mg/mL, 1mg)

Packaging Buffer:
20 mM Gly-Gly, 1 mM MgCl2, 25 mM Sucrose (pH 7.2)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human SMO cDNA (GenBank Accession Number: NM_005631.3) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Data Sheet

Background: The human smoothened homolog SMO strongly resembles that of the frizzled family of proteins, the receptors of the Wnt signaling pathway, which share several features with the Hedgehog pathway. Most often SMO functions during embryonic development, in processes such as digit patterning in the chick limb bud and left–right asymmetry of vertebrate embryos. In addition, SMO function is fundamental for the maintenance of tissue homeostasis in adults, and deregulated SMO signaling is implicated in tumorogenesis.

Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

Reference:

Ruiz-Gómez et al. (2007) The cell biology of Smo signaling and its relationships with GPCRs. Biochim Biophys Acta 1768:901-912.

FOR RESEARCH USE ONLY.
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