MULTISCREEN™ MEMBRANE PREPARATION

HUMAN RECOMBINANT NOP (OPRL1) RECEPTOR

Product Information

Catalog Number:
MC1354a

Lot Number:
MC1354a-07202012

Quantity:
1 vial (8.9 mg/ml)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human OPRL1 cDNA (GenBank Accession Number NM_000913.3) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Data Sheet

Background: NOP, ORL1 or OPRL1 (opioid receptor-like 1 receptor) is also known as nociceptin receptor, orphanin FQ receptor and KOR-3 (kappa 3-related opioid receptor). It is a receptor for the neuropeptide nociceptin/orphanin FQ and has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. In vivo experiments have demonstrated that nociceptin modulates a variety of biological functions including nociception, food intake, memory processes, cardiovascular and renal functions, spontaneous locomotor activity, gastrointestinal motility, anxiety and neurotransmitter release at peripheral and central sites.

Application: [35S]GTPγS Binding Assay

Figure 1. Dose-dependent [35S]GTPγS Binding to NOP membrane upon treatment with agonist using homogenous proximity assay method. Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Calo et al. (2000) Pharmacology of nociceptin and its receptor: a novel therapeutic target. Br J Pharmacol 129:1261-1283.

Ciccocioppo et al. (2003) The nociceptin/orphanin FQ/NOP receptor system as a target for treatment of alcohol abuse: a review of recent work in alcoholpreferring rats. Physiol Behav 79:121-128.

FOR RESEARCH USE ONLY.
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