MULTISCREEN™ MEMBRANE PREPARATION

HUMAN RECOMBINANT RXFP1 RECEPTOR

Product Information

Catalog Number:
MC1287NTb

Lot Number:
MC1287NTb-010516

Quantity:
1 vial (8.8mg/mL, 1mg)

Packaging Buffer:
20mM Gly-Gly, 1mM MgCl2, 25 mM Sucrose (pH 7.2)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human RXFP1 cDNA (GenBank accession number: NM_021634)

Recommended Storage:
Store at – 80°C. Avoid repeated freeze-thaw.

Data Sheet

Background: The relaxin family peptide receptor RXFP1, also known as LGR7 or RX1, is a 757-amino acid 7 transmembrane protein. RT-PCR detected RXFP1 expression in various organs of the reproductive system along with the brain, heart, and lungs. RXFP1 is known to be important to the connective tissue cells of the breast and play a central role in endometrial deciduation.

Application: Functional assays

Figure 1. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with cAMP HiRange kit (Cisbio 62AM6PEC) using RXFP1 stable cells. Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-RXFP1 antibody. Thin line: parental cells; thick line: receptor- expressing cells.

References:

Ivell et al. (2003) Immunoexpression of the relaxin receptor LGR7 in breast and uterine tissues of humans and primates. Reprod Biol Endocrinol 1:114-114.

Hsu et al. (2002) Activation of orphan receptors by the hormone relaxin. Science 295:671-674.

FOR RESEARCH USE ONLY.
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