MULTISCREEN™ MEMBRANE PREPARATION

HUMAN RECOMBINANT M4 RECEPTOR

Product Information

Catalog Number:
MC1025-1

Lot Number:
C1025-1-07122013

Quantity:
10.65mg/ml, 1mg

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human CHRM4 cDNA (GenBank accession number NM_000741.2) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Data Sheet

Background: The human muscarinic M4 receptor is a 479-amino acid, 7- transmembrane protein. In heart muscle, M4 receptors are coupled to specific K+ currents that regulate cardiac muscle contractions. They can also be found in tissues of the central nervous system and bladder.

Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.

References:

Eglen (2006) Muscarinic receptor subtypes in neuronal and non-neuronal cholinergic function. Auton Atacoid Pharmcol 26:219-233.

Yeh et al. (2007) Atrial tachycardia induces remodeling of muscarinic receptors and their coupled potassium currents in canine left atrial and pulmonary vein cardiomyocytes. Br J Pharmacol 152:981-982.

FOR RESEARCH USE ONLY.
All rights reserved. No part of this document may be reproduced in any form without prior permission in writing.

26219 Eden Landing Road Hayward, CA 94545 | (510) 887-0817 | info@multispaninc.com