MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT BB2 RECEPTOR
1 vial (4 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GRPR cDNA (GenBank accession number NM_005314.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
1-2 days after thawing
Background: The bombesin receptor BB2 (or gastrin-releasing peptide receptor GRPR) is responsible for many physiological actions such as inhibition of feeding, smooth muscle contraction, exocrine and endocrine secretions, thermoregulation, blood pressure and sucrose regulations, and cell growth. BB2 is expressed in the brain, as well as in colon, lung, and prostate cancer cells. The development of potent receptor antagonists that block BB2 receptor responses has potential for new therapeutic treatments in cancer.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Benya et al. (1995) Expression and characterization of cloned human bombesin receptors. Mol Pharmacol 47:10-20.
Ohki-Hamazaki et al. (2005) Development and function of bombesin-like peptides and their receptors. Int J Dev Biol 49:293-300.
Xiao et al. (2001) The human gastrin-releasing peptide receptor gene structure, its tissue expression and promoter. Gene 264:95-103.