MULTISCREEN™ DIVISION ARRESTED CELL LINE

RAT RECOMBINANT MOR RECEPTOR

Product Information

Catalog Number:
DCr1350-1a

Lot Number:
DCr1350-1a-071414

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO dhfr-

Transfection:
Full-length rat Oprm1 opioid receptor cDNA (GenBank Accession Number L13069)

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: Alpha-MEM, 10% FBS

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: Mu opioid receptor (MOR) is a receptor for beta-endorphin, inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. MOR signaling and regulation are strongly agonist-dependent. MOR mediates positive reinforcement following direct (morphine) or indirect (alcohol, cannabinoids, nicotine) activation. MOR plays a genetic role in the control of gut inflammation. MOR-deficient mice are highly susceptible to colon inflammation, with a 50% mortality rate occurring 3 days after administration of TNBS that induces inflammation. MOR agonists regulate cytokine production and T cell proliferation and might be new therapeutic molecules in inflammatory bowel disease.

Application: Functional assays

Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).

References:

Chen et al. (1993) Molecular cloning and functional expression of a mu-opioid receptor from rat brain. Mol Pharmacol 44:8-12.

Contet et al. (2004) Mu opioid receptor: a gateway to drug addiction. Curr Opin Neurobiol 14:370-378.

Philippe et al. (2003) Anti-inflammatory properties of the mu opioid receptor support its use in the treatment of colon inflammation. J Clin Invest 111:1329-1338.

FOR RESEARCH USE ONLY.
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