MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT M4 RECEPTOR

Product Information

Catalog Number:
DCG1025-1

Lot Number:
DCG1025-1-051518

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Cellbanker 2 (Amsbio 11891)

Host cell:
CHO-K1 Gqαi5

Transfection:
Expression vector containing full-length human CHRM4 cDNA (GenBank accession number NM_000741.2) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium:
DMEM/F12, 10% FBS, 10 μg/mL puromycin

Stability:
1-2 days after thawing

Data Sheet

Background: The human muscarinic M4 receptor is a 479-amino acid, 7-transmembrane protein. In heart muscle, M4 receptors are coupled to specific K+ currents that regulate cardiac muscle contractions. They can also be found in tissues of the central nervous system and bladder.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Eglen (2006) Muscarinic receptor subtypes in neuronal and non-neuronal cholinergic function. Auton Atacoid Pharmcol 26:219-233.

Yeh et al. (2007) Atrial tachycardia induces remodeling of muscarinic receptors and their coupled potassium currents in canine left atrial and pulmonary vein cardiomyocytes. Br J Pharmacol 152:981-982.

FOR RESEARCH USE ONLY.
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