MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT CB1 RECEPTOR

Product Information

Catalog Number:
DC1229-1a

Lot Number:
DC1229-1a-021012

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human CB1 cDNA (GenBank Accession Number: NM_016083) with FLAG tag sequence at N-terminus.

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: Cannabinoid Receptor 1, CNR1 also known as CB1, is involved in cannabinoid induced CNS effects. It acts by inhibiting intracellular adenylate cyclase activity and could be a receptor for anandamide. CNR1 is a potential target for the development of novel therapeutic drugs in the treatment of various conditions, such as pain, feeding disorders, vascular disease, Parkinson’s disease, and other central nerve system disorders.

Application: Functional assays

Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).

References:

Gerard, C., C. Mollereau, et al. (1990). “Nucleotide sequence of a human cannabinoid receptor cDNA.” Nucleic Acids Res 18(23): 7142

Mendizabal, V. E. and E. Adler-Graschinsky (2003). “Cannabinoid system as a potential target for drug development in the treatment of cardiovascular disease.” Curr Vasc Pharmacol 1 (3): 301-13

FOR RESEARCH USE ONLY.
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