MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT GPR84 RECEPTOR

Product Information

Catalog Number:
DC1137-1

Lot Number:
C1137-1-020312

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length human GPR84 cDNA (GenBank accession Number NM_020370) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DME/F12, 10% FBS

Stability:
stable for 1-2 days after thawing

Data Sheet

Background: Human GPR84 is a G protein-coupled receptor with 396 amino acids. The hgpr84 transcript was found in brain, heart, muscle, colon, thymus, spleen, kidney, liver, intestine, placenta, lung and leukocytes. A study in GPR84-deficient mice revealed a novel role for GPR84 in regulating early IL-4 gene expression in activated T cells in response to CD3 crosslinking.

Application: Functional assays

Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).

References:

Wittenberger et al. (2001) An expressed sequence tag (EST) data mining strategy succeeding in the discovery of new G-protein coupled receptors. J Mol Biol 307:799- 813.

Venkataraman and Kuo (2005) The G-protein coupled receptor, GPR84 regulates IL-4 production by T lymphocytes in response to CD3 crosslinking. Immunol Lett 101:144- 153.

FOR RESEARCH USE ONLY.
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