MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT GPR84 RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GPR84 cDNA (GenBank accession Number NM_020370) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DME/F12, 10% FBS
stable for 1-2 days after thawing
Background: Human GPR84 is a G protein-coupled receptor with 396 amino acids. The hgpr84 transcript was found in brain, heart, muscle, colon, thymus, spleen, kidney, liver, intestine, placenta, lung and leukocytes. A study in GPR84-deficient mice revealed a novel role for GPR84 in regulating early IL-4 gene expression in activated T cells in response to CD3 crosslinking.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
Wittenberger et al. (2001) An expressed sequence tag (EST) data mining strategy succeeding in the discovery of new G-protein coupled receptors. J Mol Biol 307:799- 813.
Venkataraman and Kuo (2005) The G-protein coupled receptor, GPR84 regulates IL-4 production by T lymphocytes in response to CD3 crosslinking. Immunol Lett 101:144- 153.