MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT GPR55 RECEPTOR
1 vial (4 x 106) frozen cells
Cellbanker 2 (Amsbio 11891)
Full-length Human GPR55 cDNA (GenBank Accession Number NM_005683.3) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable for 1-2 days after thawing
Background: GPR55 is a putative cannabinoid receptor. Its gene was mapped to chromosome 2q37, using fluorescence in situ hybridization (FISH), and its mRNA transcripts have been detected in the caudate nucleus and putamen. Recently, GPR55 was identified as a receptor for the bioactive lipid lysophosphatidylinositol (LPI).
Application: Functional assays
Figure Legend: Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Sawzdargo et al. (1999). Identification and cloning of three novel human G protein- coupled receptor genes GPR52, PsiGPR53 and GPR55: GPR55 is extensively expressed in human brain. Brain Res Mol Brain Res 64:193-198.
Oka et al. (2007) Identification of GPR55 as a lysophosphatidylinositol receptor. Biochem Biophys Res Commun 362:928–934.