MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT LPA2 (EDG4) RECEPTOR

Product Information

Catalog Number:
DC1050-6

Lot Number:
06/21/13

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
RH7777

Transfection:
Expression vector containing full-length human EDG4 cDNA (GenBank Accession Number NM_004720.4) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: The lysophosphatidic acid receptor LPA2 or endothelial differentiation, G-protein coupled receptor 4 (EDG-4) is expressed most abundantly in testes and peripheral blood leukocytes. It is reported to be a distinctive functional marker for ovarian carcinoma.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Huang et al. (2002) Lysophospholipid mediators of immunity and neoplasia. Biochim Biophys Acta 1582:161-167.

An et al. (1998) Characterization of a novel subtype of human G proteincoupled receptor for lysophosphatidic acid. J Biol Chem 273:7906-7910.

FOR RESEARCH USE ONLY.
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