MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT LPA2 (EDG4) RECEPTOR
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human EDG4 cDNA (GenBank Accession Number NM_004720.4) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable for 1-2 days after thawing
Background: The lysophosphatidic acid receptor LPA2 or endothelial differentiation, G-protein coupled receptor 4 (EDG-4) is expressed most abundantly in testes and peripheral blood leukocytes. It is reported to be a distinctive functional marker for ovarian carcinoma.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Huang et al. (2002) Lysophospholipid mediators of immunity and neoplasia. Biochim Biophys Acta 1582:161-167.
An et al. (1998) Characterization of a novel subtype of human G proteincoupled receptor for lysophosphatidic acid. J Biol Chem 273:7906-7910.