MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT CCK1 RECEPTOR

Product Information

Catalog Number:
DC1038

Lot Number:
DC1038-102313

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Full-length human CCKAR cDNA (GenBank accession Number NM_000730.1) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: Cholecystokinin (CCK) is a regulatory peptide hormone with many functions in the gastrointestinal tract and brain. CCK1 (also known as CCKA) receptors are present in the gall bladder, gastric smooth muscle cells and mucosa, myenteric neurons, and in the brain. CCK regulates gastric acid secretion, gastric emptying, and gall bladder motility in the gastrointestinal tract. Clinical studies show that CCK1 may be involved in many types of diseases, such as pancreatic disorders, motility disorders, tumor growth, and regulation of satiety.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Berna, MJ et al. (2007) Role of CCK/gastrin receptors in gastrointestinal/metabolic diseases and results of human studies using gastrin/CCK receptor agonists/antagonists in these diseases. Curr Top Med Chem 7(12):1211-31.

de Weerth, A et al. (1993) Molecular cloning, functional expression and chromosomal localization of the human cholecystokinin type A receptor. Biochem Biophys Res Commun 194(2):811-8.

FOR RESEARCH USE ONLY.
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