HUMAN RECOMBINANT MRGX2 RECEPTOR
MULTISCREEN™ MEMBRANE PREPARATION
1 vial (17.0 mg/mL), 1, 5, 10mg
Packaging Buffer: 20mM Gly-Gly, 1 mM MgCl2, 25mM Sucrose (pH 7.2)
Expression vector containing full-length human MRGX2 cDNA (GenBank Accession Number NM_054030) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving. Avoid repeated freeze-thaw
Background: MRGX2 (MAS-related GPR member X2) is widely known as a receptor for cortistatin. It is probably involved in the function of nociceptive neurons and regulation of nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14, a high potency agonist at the receptor, has biological functions including sleep regulation, locomotor activity and cortical function. Recent studies have found that MrgX2 is also a potential human PAMP-12 receptor that acts by inhibiting of the forskolin-elevated cAMP accumulation and regulates catecholamine secretion from adrenal glands.
Application: Functional assays
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Robas et al. (2003) MrgX2 is a high potency cortistatin receptor expressed in dorsal root ganglion. J Biol Chem 278:44400-44404.
Kamohara et al. (2005) Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides. Biochem Biophys Res Commun 330:1146-1152.