HUMAN RECOMBINANT MC1 RECEPTOR
MULTISCREEN™ DIVISION ARRESTED CELL LINE
1 vial (4 x 106) frozen cells
CellBanker 2 (Amsbio 11891)
Full-length Human MC1R cDNA (GenBank Accession Number NM_002386.3) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Background: The melanocortin 1 receptor (MC1R), also known as melanocyte-stimulating hormone receptor (MSHR) or melanotropin receptor, is a seven transmembrane G-protein coupled receptor expressed on the surface of melanocytes MC1 receptors binds to a class of pituitary peptide hormones known as melanocortins. Binding of melanocortins to five subtypes of melanocortin receptors exert a large array of central and peripheral effects. MC1R is one of the key proteins expressed in skin and hair and hair color. Activation of MC1R by agonist stimulates the synthesis of eumelanin by a process melanogenesis, primarily activating adenylate cyclase. MC1R has a potential role in anti-inflammatory and melanoma treatment. Further studies of the MC1 receptor are essential in gaining a better understanding of the mechanism and in vivo regulation of pigmentation and anti-inflammatory functions.
Application: Functional assays
Figure 1. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with MULTISCREENTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
Beaumont KA, et al (2010) Melanocortin MC1 receptor in human genetics and model systems. European Journal of Pharmacology 660:103-10
Neumann AG, et al. (2001) MC(1) receptors are constitutively expressed on leucocyte subpopulations with antigen presenting and cytotoxic functions. Clin Exp Immunol 126(3):441-6.