RAT RECOMBINANT KAPPA OPIOID RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length rat Oprk1 cDNA (GenBank Accession Number L22001)
Liquid nitrogen upon receiving
Propagation Medium: Alpha-MEM, 10% FBS, 800 µg/mL G418
Stable after minimum of two months continuous growth
Background: Kappa opioid receptor (KOR) is a receptor for dynorphins. KOR inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance and may play a role in arousal and regulation of autonomic and neuroendocrine functions. Some studies suggest that the stimulation of kappa opioid receptors improves memory dysfunctions resulting from the blockade of muscarinic M1 receptors. In addition, there is evidence that kappa opioid receptor agonists attenuate several behavioral responses induced by drugs of abuse, raising the possibility that kappa opioid receptor agonists may be useful for the treatment of dependence on drugs of abuse.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Cells were transiently transfected with Gαqi5. Figure 2. Dose-dependent inhibition of forskolin- stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
Ukai et al. (1995) Kappa-Opioid receptor agonists improve pirenzepine-induced disturbance of spontaneous alternation performance in the mouse. Eur J Pharmacol 281:173-178.
Hasebe et al. (2004) Possible pharmacotherapy of the opioid kappa receptor agonist for drug dependence. Ann N Y Acad Sci 1025:404-413.