HUMAN RECOMBINANT IP1 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C- 6164)
Expression vector containing full-length human PTGIR cDNA (GenBank Accession Number: NM_000960) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM-F12, 10% FBS, 10 μg/mL puromycin
Stable after minimum two months continuous growth
Background: The human prostaglandin I2 receptor IP1 (or PTGIR) mediates the actions of prostaglandin I2 (PGI2 or prostacyclin), which is a labile metabolite of arachidonic acid produced in concert with the bis-enoic prostaglandins via the cyclooxygenase pathway. PGI2 plays a major physiologic role as a strong mediator of vasodilation and inhibitor or platelet activation, and is an antithrombotic agent in vivo and mediates inflammation and pain. PGI2 derived from COX2 plays a critical role in regulating the release of rennin and consequently in renovascular hypertension.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent stimulation of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR- FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.
Boie et al. (1994) Cloning and expression of a cDNA for the human prostanoid IP receptor. J Biol Chem 269:12173-12178.
Coleman et al. (1994) VIII International union of pharmacology classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes. Pharm Rev 46:205-229.