Product Information
Catalog Number:
CG0001
Lot Number:
CG0001-031915
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
HEK293T Gαqi5
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stability:
Stable for a minimum of 2 months in continuous culture
Data Sheet
Background: Transmembrane Protein 175 (TMEM175) is an organelle specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. TMEM175 is reported to regulate lumenal pH stability and is required for autophagosome-lysosome fusion. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).