HUMAN RECOMBINANT GPR87 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GPR87 cDNA (GenBank accession number NM_023915.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% Dialyzed FBS, 3 μg/mL puromycin
Stable after minimum two months continuous growth
Background: GPR87 is a recently discovered LPA receptor. It is highly expressed in neoplastic cells found in the cervical squamous, urinary bladder carcinomas, lung carcinomas, head and neck squamous cell carcinomas, and the placenta. GPR87 plays an important role in normal ovarian development as well as intracellular signal transduction. GPR87 has been linked to lung and ovarian cancer, as well as tumor cell survival. Current research strongly supports the role of GPR87 as a p53 regulator and its potential as a therapeutic target for the treatment of cancer.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Rani, Mukta, et al. “Computational Analysis of the 3-D structure of Human GPR87 Protein: Implications for Structure-Based Drug Design.” Asian Pacific Journal of Cancer Prevention 14 (2013): 7473-82.
Gugger, Mathias, et al. “GPR87 is an overexpressed G-protein coupled receptor in squamous cell carcinoma of the lung.” Disease Markers 24 (2008): 41-50.
Zhang, Yanhong, Ariane Scoumanne, and Xinbin Chen. “G Protein-Coupled Receptor 87: a Promising Opportunity for Cancer Drug Discovery.” Mol Cell Pharmacol. 2 (2010): 111-16.