HUMAN RECOMBINANT GPR68 RECEPTOR
MULTISCREEN™ DIVISION ARRESTED CELL LINE
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GPR68 cDNA (GenBank Accession Number NM_003485) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable for 1 -2 days after thawing
Background: GPR68, also known as ovarian cancer G-protein-coupled receptor 1 (OGR1), was previously considered a receptor for sphingosylphosphorylcholine. Recent studies revealed that GPR68 is a proton-sensing GPCR that plays a major role in pH homeostasis. The receptor is expressed in the kidney, placenta, heart, lung, brain, spleen, testis, small intestines, and peripheral blood leucocytes. GPR68 stimulates inositol phosphate (IP) production, Ca2+ mobilization, and cAMP accumulation. The receptor is involved in cell-mediated responses to acidosis in the bone, acts as a metastasis suppressor gene in prostate cancer, and is a potential therapeutic target for obstructive lung diseases.
Application: Functional assays
Figure 1. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
Ludwig, M.-G., Vanek, M., Guerini, D., Gasser, J. A., Jones, C. E., Junker, U., Hofstetter, H., Wolf, R. M., Seuwen, K.Proton-sensing G-protein-coupled receptors. Nature 425: 93-98, 2003.