HUMAN RECOMBINANT GPR65 RECEPTOR CRISPR KNOCK OUT
MULTISCREENTM HETEROZYGOUS STABLE CELL LINE
1 vial (2 x 106) frozen cells
Cell Banker 2 (Amsbio 11891)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stability: Stable for a minimum of 2 months in continuous culture
Background: GPR65 also known as T cell death-associated gene 8 (TDAG8) is a proton-sensing GPCR and plays a major role in pH homeostasis. This receptor is primarily expressed in lymphoid tissues (spleen, thymus, leukocytes and lymph nodes) and cancer tissues. The major function of this receptor is to reduce immune-mediated inflammation by regulating cytokine production from T cells and macrophages.
Figure 1. Verified GPR65 coding sequence with deletion highlighted in grey.
Figure 2: Verified GPR65 knock out protein sequence product
Ludwig, M.-G., Vanek, M., Guerini, D., Gasser, J. A., Jones, C. E., Junker, U., Hofstetter, H., Wolf, R. M., Seuwen, K. Proton-sensing G-protein-coupled receptors. Nature 425: 93-98, 2003.
Saxena, H., Deshpande, D., Tiegs, B., Yan, H., Battafarano, R., Burrows, W., Penn, R. (2012). The GPCR OGR1 (GPR68) mediates diverse signalling and contraction of airway smooth muscle in response to small reductions in extracellular pH. British Journal of Pharmacology, 166(3), 981–990.
Satoshi Ishii, Yasuyuki Kihara and Takao Shimizu (2005) Identification of T Cell Death- associated Gene 8 (TDAG8) as a Novel Acid Sensing G-protein-coupled Receptor. J Biol Chem 280: 9083-9087