HUMAN RECOMBINANT GPR45 RECEPTOR
MULTISCREEN™ STABLE CELL LINE
1 vial (2 x 106) frozen cells
Cell Banker 2 (Amsbio 11891)
Full-length Human GPR45 (GenBank Accession Number NM_007227.3) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin, 250 μg/mL hygromycin
Stability: In progress
Background: GPR45 is an Orphan-A GPCR and an orthologue of a putative lysophosphatidic acid receptor. Its expression is found in brain: frontal cortex and caudate, but not in thalamus, hippocampus or putamen. This protein may function in the central nervous system. In addition, this expression is documented in human liver, and in mouse brain as well.
Application: Functional assays
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Black line: parental cells; gray line: receptor-expressing cells.
Kawasawa Y, Kume K, Nakade S, Haga H, Izumi T, Shimizu T. (2000) Brain-specific expression of novel G-protein-coupled receptors, with homologies to Xenopus PSP24 and human GPR45. Biochem Biophys Res Commun, 276 (3): 952-6. [PMID:11027574]