HUMAN RECOMBINANT GPR151 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Cell Banker 2 (Amsbio 11891)
CHO-K1 Gα16 Gqi5
Full-length Human GPR151 (GenBank Accession Number NM_194251) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 10 μg/mL puromycin, 250 µg/mL hygromycin, 1 mg/mL G418
Background: The diverse physiological effects of Galanin, a biologically active neuropeptide, are mediated through cell surface G protein-coupled receptors. There are three galanin receptor subtypes, GALR1, GALR2 and GALR3 that have been widely used and Recently, a new GPCR, GalRL4 have been identified, termed as GPR151, which shows 41–43% similarity at the amino-acid level with the galanin-receptor subfamily. Galanin, widely distributed in the central and peripheral nervous systems and the endocrine systems, binds to galanin receptors to induce several regulatory functions in neuronal cells, including neuroregeneration, control of endocrine and exocrine secretions, and modulation of sensory and behavioral functions. Galanin agonists have been shown to have therapeutic application in treatment of chronic pain; galanin antagonists have therapeutic potential in treatment of Alzheimer’s disease, depression, and feeding disorders.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti- FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Branchek et al. (1998) Molecular biology and pharmacology of galanin receptors. Ann N Y Acad Sci 863: 94-107.
Wang et al. (1998) Differential intracellular signaling of the GalR1 and GalR2 galanin receptor subtypes. Biochemistry 37:6711-6717.
Ignatov et al. (2004) Cloning and characterization of a novel G-protein-coupled receptor with homology to galanin receptors.Neuropharmacology, 46 (8): 1114-20.