Product Information
Catalog Number:
C1069
Lot Number:
C1069-012722
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Cellbanker 2 (Amsbio)
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human GPR15 cDNA (GenBank Accession Number NM_005290.4)
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin
Recommended Storage:
Liquid nitrogen upon receiving
Stability: Stable in continuous culture for a minimum of 2 months
Data Sheet
Background: GPR15 is a class A orphan G protein-coupled receptor found in epithelial cells, synovial macrophages, endothelial cells and lymphocytes especially T cells. Studies have accumulated evidence that GPR15 may modulate chronic inflammation. Findings also have implicated GPR15 for potential therapeutic intervention.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MULTISCREENTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Ocón, B., Pan, J., Dinh, T. T., Chen, W., Ballet, R., Bscheider, M., Habtezion, A., Tu, H., Zabel, B. A., & Butcher, E. C. (2017). A mucosal and cutaneous chemokine ligand for the lymphocyte chemoattractant receptor GPR15. Frontiers in Immunology, 8.
Suply, T., Hannedouche, S., Carte, N., Li, J., Grosshans, B., Schaefer, M., Raad, L., Beck, V., Vidal, S., Hiou-Feige, A., Beluch, N., Barbieri, S., Wirsching, J., Lageyre, N., Hillger, F., Debon, C., Dawson, J., Smith, P., Lannoy, V., Bassilana, F. (2017). A natural ligand for the orphan receptor GPR15 modulates lymphocyte recruitment to epithelia. Science Signaling, 10(496).
Hayn, M., Blötz, A., Rodríguez, A., Vidal, S., Preising, N., Ständker, L., Wiese, S., Stürzel, C. M., Harms, M., Gross, R., Jung, C., Kiene, M., Jacob, T., Pöhlmann, S., Forssmann, W.- G., Münch, J., Sparrer, K. M., Seuwen, K., Hahn, B. H., & Kirchhoff, F. (2021). Natural cystatin C fragments inhibit GPR15-mediated HIV and SIV infection without interfering with GPR15L signaling. Proceedings of the National Academy of Sciences, 118(3).