HUMAN RECOMBINANT GPR119 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human GPR119 cDNA (GenBank Accession Number NM_178471.1) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Background: GPR119 is a G protein-coupled receptor for the endogenous lipid signaling agent oleoylethanolamide (OEA). GPR119 is predominantly expressed in the pancreas (beta- cells) and gastrointestinal tract (enteroendocrine cells). In vitro studies have indicated a role for GPR119 in the modulation of insulin release by pancreatic beta-cells and of GLP-1 secretion by gut enteroendocrine cells.
Application: Functional assays
Figure 1. Dose-dependent accumulation of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.
Hilary et al. (2006) Deorphanization of a G protein-coupled receptor for oleoylethanolamide and its use in the discovery of smallmolecule hypophagic agents. Cell Metabolism 3:167-175.
Overton et al. (2007) GPR 119, a novel G protein-coupled receptor target for the treatment of type 2 diabetes and obesity. Br J Pharmacol 153 suppl 1:S76-S81.