HUMAN RECOMBINANT FSH RECEPTOR
MULTISCREEN™ STABLE CELL LINE
1 vial (2 x 106) frozen cells
Cell Banker 2
Expression vector containing full-length human FSHR cDNA (GenBank Accession Number NM_000145)
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 ug/mL puromycin
Stability: In progress
Background: Follicle stimulating hormone (FSH) is a gonadotropin hormone secreted by anterior pituitary gonadotropic cells. This glycoprotein plays an important role in: growth, development, pubertal maturation, and reproductive physiology. The FSH receptor (FSHR) is a G protein–coupled receptor that in response to FSH mediates estrogen synthesis and follicular growth in response to FSH.
Application: Functional assays
Figure 1. Dose-dependent stimulation of intracellular cAMP level upon treatment with ligand, measured with MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Haldar et al. (2022). Overview of follicle stimulating hormone and its receptors in reproduction and in stem cells and cancer stem cells. Int J Biol Sci. Jan 1;18(2):675- 692
Stilley and Segaloff (2018). FSH Actions and Pregnancy: Looking Beyond Ovarian FSH Receptors. Endocrinology. Dec 1;159(12):4033-4042.
Bhartiya and Patel (2021). An overview of FSH-FSHR biology and explaining the existing conundrums. J Ovarian Res. Oct 30;14(1):144.