HUMAN RECOMBINANT FPR2 RECEPTOR
MULTISCREENTM DIVISION ARRESTED CELL LINE
1 vial (4 x 106) frozen cells
Cellbanker 2 (AmsBio 11891)
Expression vector containing full-length human FPR2 cDNA (GenBank Accession Number NM_001462.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Background: The lipoxin A4 receptor ALX is also known as formyl peptide receptor- like 1 (FPRL1) or formyl peptide receptor 2 (FPR2). It shares 69% amino acid identity with FPR but displays low affinity for bacterial peptide N-formyl-methionyl- leucyl-phenylalanine (fMLF). ALX is highly expressed on neutrophils and monocytes and mediates cell chemotaxis. By interacting with a variety of exogenous and host- derived agonists, ALX may have important implications in the pathogenesis of human diseases such as HIV, Alzheimer’s disease (AD), amyloidosis and prion diseases.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
Le et al. (2002) Formyl-peptide receptors revisited. Trends Immunol 23:541-548.
Migeotte et al. (2005) Identification and characterization of an endogenous chemotactic ligand specific for FPRL2. J Exp Med 201:83-93.