Product Information
Catalog Number:
DC1205
Lot Number:
DC1205-100623
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium:
Cell Banker 2
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human PTGFR cDNA (GenBank accession number NM_000959) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Data Sheet
Background: Prostaglandin F (2-alpha) is involved in a number of physiologic processes. It serves as a potent luteolytic agent in many species, has been implicated as a modulator of intraocular pressure, smooth muscle contraction in the uterus and elsewhere. Its effects on cells are mediated through specific interaction with the prostaglandin F receptor (FP or PTGFR). Knockout mice lacking the FP receptor are unable to deliver normal fetuses at term due to a lack of response to oxytocin. The mice also failed to show the decline in serum progesterone expected to precede parturition.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
References:
Abramovitz et al. (1994) Cloning and expression of a cDNA for the human prostanoid FP receptor. J Biol Chem 269(4):2632-6.
Kim et al. (2012) Prostaglandin FP receptor inhibitor reduces ischemic brain damage and neurotoxicity. Neurobiol Dis 48(1):58-65.