HUMAN RECOMBINANT D4 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length Human DRD4 cDNA (GenBank Accession NM_000797) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 250 μg/mL hygromycin, 1μg/mL puromycin
Stable in culture for minimum of two months
Background: The human dopamine receptor D4 is a D2-like receptor that inhibits adenylyl cyclase activity and activates K+ channels. D4 receptor antagonists show great potential in the treatment of human personality and psychiatric disorders, such as ADHD (Attention deficit hyperactivity disorder), schizophrenia, alcoholism, and drug addiction.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Kulkarni SK, et al. (2000) Dopamine D4 receptors and development of newer antipsychotic drugs. Fundam Clin Pharmacol 14(6):529-39.
Missale C, et al. (1998) Dopamine receptors: from structure to function. Physiol Rev 78(1):189-225.
Van Tol HH, et al. (1991) Cloning of the gene for a human dopamine D4 receptor with high affinity for the antipsychotic clozapine. Nature 350(6319):610-4