HUMAN RECOMBINANT CXCR7 RECEPTOR
MULTISCREEN™ β-ARRESTIN2 STABLE CELL LINE
Catalog Number: CA1150-BA2
Lot Number: CA1150-BA2-080921
Quantity: 1 vial (2 x 106) frozen cells
Freeze Medium: Cellbanker 2 (Amsbio)
Host cell: HEK293T β-Arrestin2
Transfection: Expression vector containing full-length human CXCR7 cDNA (GenBank Accession Number: NM_020311.2) with FLAG tag sequence at N-terminus and ARRB2 cDNA (GenBank Accession Number NM_004313.3)
Recommended Storage: Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin, 50 μg/mL hygromycin
Stability: In progress
Background: CXCR7 (or RDC1) is a recently deorphanized G-protein coupled receptor which binds with high affinity the inflammatory and homing chemokines CXCL11/ITAC and CXCL12/SDF-1. CXCR7 is expressed in bladder, spleen, heart, skeletal muscle, peripheral nervous system and placenta. CXCR7 does not mediate typical chemokine receptor responses such as leukocyte trafficking. Recent findings in zebrafish indicate that a critical activity of the receptor is scavenging of CXCL12 thereby generating guidance cues for CXCR4-dependent migration.
Application: Functional assays
Figure 1. Dose-dependent stimulation from arrestin recruitment upon treatment with ligand, measured with MultiScreenTM β-Arrestin Assay Kit (Multispan MSBA01).
Sreedharan et al. (1991) Cloning and expression of the human vasoactive intestinal peptide receptor. Proc Natl Acad Sci USA 88:4986-4990.
Shimizu et al. (1991) A putative G protein-coupled receptor, RDC1, is a novel coreceptor for human and simian immunodeficiency viruses. Virol 74:619-626.