HUMAN RECOMBINANT CXCR7 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CXCR7 cDNA (GenBank Accession Number: NM_020311.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Background: CXCR7 (or RDC1) is a recently deorphanized G-protein coupled receptor which binds with high affinity the inflammatory and homing chemokines CXCL11/ITAC and CXCL12/SDF-1. CXCR7 is expressed in bladder, spleen, heart, skeletal muscle, peripheral nervous system and placenta. CXCR7 does not mediate typical chemokine receptor responses such as leukocyte trafficking. Recent findings in zebrafish indicate that a critical activity of the receptor is scavenging of CXCL12 thereby generating guidance cues for CXCR4-dependent migration.
Figure 1. Receptor Expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.
Sreedharan et al. (1991) Cloning and expression of the human vasoactive intestinal peptide receptor. Proc Natl Acad Sci USA 88:4986-4990.
Shimizu et al. (1991) A putative G protein-coupled receptor, RDC1, is a novel coreceptor for human and simian immunodeficiency viruses. Virol 74:619-626.