Product Information
Catalog Number:
C1003
Lot Number:
C1003-031506
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human CXCR3 cDNA (GenBank Accession Number: NM_001504) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability:
Stable in culture for minimum of two months
Data Sheet
Background: CXCR3 (chemokine receptor 3) is a receptor for SCYB9/MIG, SCYB10/INP10 and SCYB11/ITAC. The chemokines that target CXCR3 in concert with Th1 cytokine appear to play a beneficial role to atopic disorders including a range of conditions such as allergic asthma, -rhinitis, -conjunctivitis, -dermatitis, food and drug allergies and anaphylaxis.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor Expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Loetscher, M., B. Gerber, et al. (1996). “Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes.” J Exp Med 184(3): 963-9.
Arimilli, S., W. Ferlin, et al. (2000). “Chemokines in autoimmune diseases.” Immunol Rev 177: 43-51.