Product Information
Catalog Number:
H1231
Lot Number:
H1231-062510
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
HEK293T
Transfection:
Full-length Human CALCR cDNA (GenBank Accession Number NM_001742) with FLAG-tag sequence at the N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability:
Stable in culture for minimum of two months
Data Sheet
Background: Calcitonin receptor (CT or CALCR) is a receptor for calcitonin. In the presence of the receptor activity modifying proteins (RAMP1, 2 or 3), it acts as a receptor for amylin. CT is expressed in brain, bone, stomach, intestine, kidney and testes. Data from transgenic mice support a major role for calcitonin and its receptor in the formation and resorption aspects of bone metabolism under physiological conditions.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, monitored with FlexStation. Figure 2. Dose-dependent increase of intracellular cAMP level upon treatment with ligand. Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
References:
Gorn et al. (1992) Cloning, characterization, and expression of a human calcitonin receptor from an ovarian carcinoma cell line. J Clin Invest 90:1726-1735.
Taboulet et al. (1998) Calcitonin receptor polymorphism is associated with a decreased fracture risk in post-menopausal women. Hum Mol Genet 7:2129-2133.