Product Information
Catalog Number:
C1016a
Lot Number:
C1016a-091010
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human CCR8 cDNA (GenBank Accession Number NM_005201.2) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stability:
Stable in culture for minimum of two months
Data Sheet
Background: CCR8 (C-C chemokine receptor type 8) is a receptor for the chemokines CCL1 (I-309). The receptor regulates monocyte chemotaxis and thymic cell line apoptosis, and is an additional alternative coreceptor with CD4 for HIV-1 infection. CCR8 plays a role on cell types of relevance to respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD) and chronic bronchitis. The interference of CCR8 from its ligand represents a new approach in allergy therapy.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
References:
Simmons et al. (2000) Co-receptor use by HIV and inhibition of HIV infection by chemokine receptor ligands. Immunol Rev 177:112-126.
Garcia et al. (2005) New chemokine targets for asthma therapy. Curr Allergy Asthma Rep 5:155-160