HUMAN RECOMBINANT CCR5 RECEPTOR

MULTISCREEN™ DIVISION ARRESTED CELL LINE

Product Information

Catalog Number:
DC1013b

Lot Number:
03/12/13

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human CCR5 cDNA (GenBank accession number NM_000579) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: CCR5 (chemokine (C-C motif) receptor 5) is a receptor for C-C type chemokines MIP-1-α, MIP-1-ß and CCL5. CCR5 plays a role in the control of granulocytic lineage proliferation or differentiation. It also acts as co-receptor with CD4 for primary non-syncytium- inducing strains (macrophage-tropic) of HIV-1 virus.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Cells were transiently transfected with Gqi5.

References:

Deng et al. (1996) Identification of a major co-receptor for primary isolates of HIV-1. Nature 381:661-666.

Oppermann (2004) Chemokine receptor CCR5: insights into structure, function, and regulation. Cell Signal 16:1201-1210.

FOR RESEARCH USE ONLY.
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