Product Information
Catalog Number:
DC1010
Lot Number:
DC1010-080125
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium:
Cell Banker 2
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human CCR2 cDNA (GenBank accession number NM_000647.3) with FLAG tag sequence at N-terminu
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Data Sheet
Background: CCR2 (C-C chemokine receptor type 2 or monocyte chemoattractant protein 1 receptor) is a receptor for the MCP-1, MCP-3 and MCP-4 chemokines. It is also an alternative coreceptor with CD4 for HIV-1 infection. CCR2 has been widely considered as a potential therapeutic target for autoimmune disease and inflammatory disorders of the lung, particularly rheumatoid arthritis, and various CCR2 blocking agents have been developed, some of which might have therapeutic values.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP accumulation upon treatment with ligand, measured with MULTISCREEN™ TR- FRET cAMP 1.0 No Wash Assay Kit (Multispan MCSM01).
References:
Quinones et al. (2005) The complex role of the chemokine receptor CCR2 in collagen-induced arthritis: implications for therapeutic targeting of CCR2 in rheumatoid arthritis. J Mol Med 83:672- 681.
Rose et al. (2003) Significant involvement of CCL2 (MCP-1) in inflammatory disorders of the lung. Microcirculation 10:273-288.