RAT RECOMBINANT CB2 RECEPTOR (HIGH EXPRESSION)
MULTISCREEN™ STABLE CELL LINE
1 vial (2 x 106) frozen cells
Cell Banker 2
Expression vector containing full-length rat CNR2 cDNA (GenBank Accession Number NM_001164143.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 ug/ml puromycin
Stability: In progress
Background: Cannabinoid receptor 2 (CB2) is expressed in peripheral and immune tissues. CB2 in rodents is involved not only in processes of regulation of bone homeostasis but also in cannabinoid-induced central nerve system effects. This receptor can serve as a potential therapeutic target in the treatment of various disorders affecting central nerve system or skeletal homeostasis. It was also demonstrated that CB2 receptor is involved in the reduction of chemotherapy- induced allodynia.
Application: Functional assays
Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MULTISCREENTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Grey line: parental cells; black line: receptor-expressing cells.
Gong et al. (2006) Cannabinoid CB2 receptors: immunohistochemical localization in the rat brain. Brain research, 1071(1), 10-23.
Raphael-Mizrahi and Gabet (2020). The cannabinoids effect on bone formation and bone healing. Curr. Osteoporos, 18(5), 433-438.
O’Hearn et al. (2017). Modulating the endocannabinoid pathway as treatment for peripherial neuropathic pain: a selected review of preclinical studies. Annals of palliative medicine, 6(Suppl 2), S209-S214.