DOG RECOMBINANT CB1 RECEPTOR
MULTISCREENTM STABLE CELL LINE
1 vial (2 x 106) frozen cells
Cellbanker 2 (Amsbio 11891)
Expression vector containing full-length Dog CB1 cDNA (GenBank Accession Number XM_038684131.1) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 µg/mL puromycin
Stability: In progress
Background: Cannabinoid Receptor 1, CNR1 also known as CB1, is involved in cannabinoid induced CNS effects. It acts by inhibiting intracellular adenylate cyclase activity and could be a receptor for anandamide. CNR1 is a potential target for the development of novel therapeutic drugs in the treatment of various conditions, such as pain, feeding disorders, vascular disease, Parkinson’s disease, and other central nerve system disorders.
Application: Functional assays
Figure 1. Dose-dependent stimulation of intracellular cAMP accumulation upon treatment with ligand, measured with MULTISCREENTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Mendizabal and Adler-Graschinsky (2003) Cannabinoid system as a potential target for drug development in the treatment of cardiovascular disease. Curr Vasc Pharmacol 1:301-313.
Gerard et al. (1990) Nucleotide sequence of a human cannabinoid receptor cDNA. Nucleic Acids Res 18:7142.