Product Information
Catalog Number:
H1437
Lot Number:
H1437-090814
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Sigma Freezing Medium (C-6164)
Host cell:
HEK293T
Transfection:
Expression vector containing full-length human ADRB1 cDNA (GenBank Accession Number AF169007.1) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 800 μg/mL G418
Stability:
Stable in culture for minimum of two months
Data Sheet
Background: Norepinephrine is implicated in a wide range of physiological processes through activation of nine different G-protein-coupled receptors (α1a, α1b, α1d, α2a, α2b, α2c, ß1, ß2, ß3). The human ß1-adrenergic receptor is a 477–amino acid protein found in various heart and brain tissues. ß1 has an important role in the contractile action of valves in cardiac and digestive systems.
Application: Functional assays
Figure 1. Dose-dependent stimulation of pERK level upon treatment with ligand, monitored with FlexStation. Figure 2. Dose-dependent increase of intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01). Figure 3. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.
References:
Oostendorp et al. (2000) Contribution of beta-adrenoceptor subtypes to relaxtion of colon and oesophagus and pacemaker activity of ureter in wildtype and beta(3)-adrenoceptor knockout mice. Br J Pharmacol 130:747-758.
Sato et al. (1996) Molecular characterization of pharmacological properties of T-0509 for beta-adrenoceptors. Eur J Pharmacol 315:363-367.