RAT RECOMBINANT BB1 RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Full-length rat NMBR cDNA (GenBank Accession Number NM_012799) with flag tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable after minimum of two months continuous growth
Background: The BB1 receptor (also known as NMB-R or Neuromedin B receptor) is a receptor for neuromedin-B (NMB), which is a mammalian bombesin-like peptide distributed widely in the central nervous system. The NMBR pathway is involved in the regulation of a wide variety of behaviors, such as spontaneous activity, feeding and anxiety-related behavior. A study using NMBR-deficient mice suggested that dysfunction in the NMBR pathway may constitute one of the risk factors of stress vulnerability.
Application: Functional assays
Figure legend: Dose-dependent calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Moody et al. (2000) Nonpeptide neuromedin B receptor antagonists inhibit the proliferation of C6 cells. Eur J Pharmacol 409:133-142.
Yamada et al. (2002) Restraint stress impaired maternal behavior in female mice lacking the neuromedin B receptor (NMB-R) gene. Neurosci Lett 330:163-166.
Herold et al. (2003) The neuromedin B receptor antagonist, BIM-23127, is a potent antagonist at human and rat urotensin-II receptors. Br J Pharmacol 139:203-207.