Product Information
Catalog Number:
CG1436-1
Lot Number:
CG1436-1-021522
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Cellbanker 2 (Amsbio)
Host cell:
CHO-K1 Gqi5
Transfection: Expression vector containing full-length human ADRA2C cDNA (GenBank Accession Number AY455666) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin, 250 μg/mL hygromycin
Stability: Stable for a minimum of 2 months in continuous culture
Data Sheet
Background: The human adrenergic α2c receptor is a 458-amino acid, 7- transmembrane protein. It belongs to the group of nine adrenoceptors that mediate the biological actions of the endogenous catecholamines adrenaline and noradrenaline. All three alpha2-receptor subtypes may operate as presynaptic inhibitory feedback receptors to control the release of neurotransmitters. The α2c receptor is of particular importance as it appears to regulate venous vasoconstriction.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MULTISCREENTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Black line: parental cells; gray line: receptor-expressing cells.
References:
Gyires et al. (2009) alpha(2)-Adrenoceptor subtypes-mediated physiological, pharmacological actions. Neurochem Int 55:447-453.
Knaus et al (2007) Alpha2-adrenoceptor subtypes–unexpected functions for receptors and ligands derived from gene-targeted mouse models. Neurochem Int 51:277-281.