HUMAN RECOMBINANT α2B RECEPTOR

MULTISCREENTM STABLE CELL LINE

Product Information

Catalog Number:
CG1435-1

Lot Number:
CG1435-1-021422

Quantity:
1 vial (2 x 106) frozen cells

Freeze Medium:
Cellbanker 2 (Amsbio)

Host cell:
CHO-K1 Gqi5

Transfection: Expression vector containing full-length human ADRA2B cDNA (GenBank Accession Number NM_000682.5) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin, 250 μg/mL hygromycin

Stability: Stable for a minimum of 2 months in continuous culture

Data Sheet

Background: Adrenergic α2b receptor belongs to the group of nine adrenoceptors that mediate the biological actions of the endogenous catecholamines adrenaline and noradrenaline. While α2a receptor exerts a tonic sympathoinhibitory function, the α2b is responsible for the central hypertensive sympathoexcitatory response. Development of agents capable of selectively activating the α2a or blocking the α2b may further improve our capability to treat hypertension, ischemic heart disease and heart failure. The α2b receptor also contributes to the peripheral regulation of vascular tone.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MULTISCREENTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Black line: parental cells; gray line: receptor- expressing cells.

References:

Knaus et al (2007) Alpha2-adrenoceptor subtypes–unexpected functions for receptors and ligands derived from gene-targeted mouse models. Neurochem Int 51:277-281.

Philipp et al. (2002) Physiological significance of alpha(2)-adrenergic receptor subtype diversity: one receptor is not enough. Am J Physiol Regul Integr Comp Physiol. 283:R287-R295.

FOR RESEARCH USE ONLY.
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