Product Information
Catalog Number:
CG1434-1
Lot Number:
CG1434-1-021422
Quantity:
1 vial (2 x 106) frozen cells
Freeze Medium:
Cellbanker 2 (Amsbio)
Host cell:
CHO-K1 Gqi5
Transfection: Expression vector containing full-length human ADRA2A cDNA (GenBank accession Number NM_000681.2) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin, 250 μg/mL hygromycin
Stability: Stable for a minimum of 2 months in continuous culture
Data Sheet
Background: Adrenergic α2a receptor belongs to the group of nine adrenoceptors that mediate the biological actions of adrenaline and noradrenaline. The α2a receptor is expressed in the brain, spleen, kidney, heart and gastrointestinal tract. It is centrally predominant and exerts a tonic sympathoinhibitory function, whereas peripherally it has a vasoconstrictive effect. The α2a receptor is also involved in sedation, digestive functions and analgesia.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MULTISCREENTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Figure 2. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Black line: parental cells; gray line: receptor- expressing cells.
References:
Knaus et al (2007) Alpha2-adrenoceptor subtypes–unexpected functions for receptors and ligands derived from gene-targeted mouse models. Neurochem Int 51:277-281.
Philipp et al (2002) Physiological significance of alpha(2)-adrenergic receptor subtype diversity: one receptor is not enough. Am J Physiol Regul Integr Comp Physiol 283:R287-R295.