HUMAN RECOMBINANT α1D ADRENERGIC RECEPTOR
MULTISCREEN™ STABLE CELL LINES
1 vial (2 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human ADRA1D cDNA (GenBank accession number NM_000678.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS, 10 μg/mL puromycin
Stable in culture for minimum of two months
Background: Norepinephrine is implicated in a wide range of physiological processes through activation of nine different G-protein-coupled receptors (α1a, α1b, α1d, α2a, α2b, α2c, ß1, ß2, ß3). The α1D-adrenoceptor is found mainly intracellularly. As with the other α1-ARs, α1D is used by the sympathetic nervous system to regulate systemic arterial blood pressure and blood flow. The α1-ARs also play a major role in mediating growth responses in cardiac and vascular smooth muscle cells. Studies have shown that the α1D-adrenoceptor is responsible for regulating arterial blood pressure by vasoconstriction. Knockout mice lacking the α1D gene maintained significantly lower basal systolic and mean arterial blood pressures, compared to wild type mice. Also, the contractile response of the aorta and the pressor response of isolated perfused mesenteric arterial beds to α1-AR stimulation were reduced in mice lacking α1D.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, monitored with FlexStation.
Chalothorn et al. (2002) Differences in the cellular localization and agonist-mediated internalization properties of the α1-adrenoreceptor subtypes. Mol Pharmacol 61(5):1008-1016.
Tanoue et al. (2002) The alpha(1D)-adrenergic receptor directly regulates arterial blood pressure via vasoconstriction. J Clin Invest. 109(6):765-775.
Weinberg et al. (1994) Cloning, expression and characterization of human alpha adrenergic receptors alpha 1a, alpha 1b and alpha 1c. Biochem. Biophys. Res. Commun. 201:1296-1304.