Product Information
Catalog Number:
DC1431-1a
Lot Number:
DC1431-1a-030124
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium:
Cellbanker2
Host cell:
CHO-K1
Transfection:
Expression vector containing full-length human ADRA1A cDNA (GenBank accession number NM_000680.2) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F12, 10% FBS
Data Sheet
Background: Norepinephrine is implicated in a wide range of physiological processes through activation of nine different G-protein-coupled receptors (α1a, α1b, α1d, α2a, α2b, α2c, ß1, ß2, ß3). The α1a-adrenoceptor is highly expressed in human vasculature. As with the other α1-ARs, α1a is used by the sympathetic nervous system to regulate systemic arterial blood pressure and blood flow. The α1-ARs also play a major role in cardiac and vascular smooth muscle cells. The knockout mouse models lacking the α1a-adrenergic receptors have highlighted the potential implications of this receptor subtype in variety of functions including the regulation of renal artery contractions, smooth muscle contractions, and vasoconstriction.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
References:
Chalothorn et al. (2002) Differences in the cellular localization and agonist-mediated internalization properties of the α1-adrenoreceptor subtypes. Mol Pharmacol 61(5):1008-1016.
Weinberg et al. (1994) Cloning, expression and characterization of human alpha adrenergic receptors alpha 1a, alpha 1b and alpha 1c. Biochem. Biophys. Res. Commun. 201:1296-1304.
Hague et al. (2003) a1-Adrenergic receptor subtypes: non-identical triplets with different dancing partners? Life Sciences 74:411-418.