HUMAN RECOMBINANT 5-HT6 RECEPTOR

MULTISCREEN™ DIVISION ARRESTED CELL LINE

Product Information

Catalog Number:
DC1331-1

Lot Number:
DC1331-1-011823

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
CellBanker 2

Host cell:
CHO-K1

Transfection:
Expression vector containing full-length Human HTR6 cDNA (GenBank Accession Number NM_000871) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM/F-12, 10% FBS

Data Sheet

Background: Human 5-HT6 receptors are G protein-coupled receptors. The cDNA encodes a 440-amino-acid polypeptide. Human 5-HT6 receptors are G protein- coupled receptors. The cDNA encodes a 440-amino-acid polypeptide. The 5-HT6 receptors are expressed exclusively in brain regions such as striatum, cortex, nucleus accumbens, olfactory tubercle, hippocampus, hypothalamus, amygdala and cerebellum. The 5-HT6 receptors appear to regulate GABAergic, glutamatergic and cholinergic neurotransmission and are involved in the regulation of cognition, feeding and sleep/wake regulation. They may also play a role in mediating the effects of some antipsychotic agents, and in modulating sensory gating which is often affected in schizophrenic patients.

Application: Functional assays

Figure Legend: Dose-dependent stimulation of intracellular cAMP level upon treatment with ligand, measured with MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).

References:

González-Vera, J., Medina, R., Martín-Fontecha, M. et al. (2017). A new serotonin 5-HT6 receptor antagonist with procognitive activity –Importance of a halogen bond interaction to stabilize the binding. Sci Rep 7, 41293.

Woolley ML, Marsden CA, and Fone KC (2004) 5-HT6 receptors. Curr Drug Targets CNS Neurol Disord 3:59-79.

Wesołowska A (2002) In the search for selective ligands of 5-HT5, 5-HT6 and 5-HT7 serotonin receptors. Pol J Pharmacol 54:327-341.

FOR RESEARCH USE ONLY.
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