Product Information
Catalog Number:
DC1331-1
Lot Number:
DC1331-1-011823
Quantity:
1 vial (4 x 106) frozen cells
Freeze Medium:
CellBanker 2
Host cell:
CHO-K1
Transfection:
Expression vector containing full-length Human HTR6 cDNA (GenBank Accession Number NM_000871) with FLAG tag sequence at N-terminus
Recommended Storage:
Liquid nitrogen upon receiving
Propagation Medium: DMEM/F-12, 10% FBS
Data Sheet
Background: Human 5-HT6 receptors are G protein-coupled receptors. The cDNA encodes a 440-amino-acid polypeptide. Human 5-HT6 receptors are G protein- coupled receptors. The cDNA encodes a 440-amino-acid polypeptide. The 5-HT6 receptors are expressed exclusively in brain regions such as striatum, cortex, nucleus accumbens, olfactory tubercle, hippocampus, hypothalamus, amygdala and cerebellum. The 5-HT6 receptors appear to regulate GABAergic, glutamatergic and cholinergic neurotransmission and are involved in the regulation of cognition, feeding and sleep/wake regulation. They may also play a role in mediating the effects of some antipsychotic agents, and in modulating sensory gating which is often affected in schizophrenic patients.
Application: Functional assays
Figure Legend: Dose-dependent stimulation of intracellular cAMP level upon treatment with ligand, measured with MULTISCREEN™ TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).
References:
González-Vera, J., Medina, R., Martín-Fontecha, M. et al. (2017). A new serotonin 5-HT6 receptor antagonist with procognitive activity –Importance of a halogen bond interaction to stabilize the binding. Sci Rep 7, 41293.
Woolley ML, Marsden CA, and Fone KC (2004) 5-HT6 receptors. Curr Drug Targets CNS Neurol Disord 3:59-79.
Wesołowska A (2002) In the search for selective ligands of 5-HT5, 5-HT6 and 5-HT7 serotonin receptors. Pol J Pharmacol 54:327-341.