HUMAN RECOMBINANT 5-HT4 RECEPTOR
MULTISCREEN™ DIVISION-ARRESTED CELL LINE
1 vial (4 x 106) frozen cells
Cellbanker 2 (AMSBio, Cat# 11891)
Expression vector containing full-length human HTR4 cDNA (GenBank accession number NM_000870.4) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS, 1 μg/mL puromycin
Stable for 1-2 days after thawing
Background: The human serotonin receptor 5-HT4 is a G protein-coupled receptor. 5-HT4 receptors can be found in the central nervous system and in peripheral tissues such as the alimentary tract, urinary bladder, heart and adrenal gland. 5-HT4 ligands may prove to be therapeutic in the treatment of disorders such as cardiac arrhythmias, irritable bowel syndrome and urinary incontinence.
Application: Functional assays
Figure Legend: Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor- expressing cells.
Talley (2003) Evaluation of drug treatment in irritable bowel syndrome. Br J Clin Pharmacol 56:362-369.
Van den Wyngaert et al. (1997) Cloning and expression of a human serotonin 5-HT4 receptor DNA. J Neurochem 69:1810-1819.